کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5157209 1500589 2017 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Biomacromolecular-based ionic-covalent hydrogels for cell encapsulation: The atelocollagen − Oxidized polysaccharides couples
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Biomacromolecular-based ionic-covalent hydrogels for cell encapsulation: The atelocollagen − Oxidized polysaccharides couples
چکیده انگلیسی
Mixed crosslinked networks of ionic-covalent entanglement type were prepared starting from ternary mixtures of atelocollagen (aK; as fibrillary matrix generator), sodium hyaluronate (NaHyal; a microfibrillation assistant), and oxidized polysaccharides (OxPolys; as both cross-linkers and matrix fillers), and were tested as hydrogels for eukaryotic cell encapsulation. Either oxidized gellan (GellOx) or pullulan (PullOx) were used. An original procedure and optimal hydrogel recipes were developed to encapsulate fibroblasts and adipose-derived stem cells, while preserving their viability and proliferative ability during ex vivo temporarily storage. Physical-chemical, rheological, and biocompatibility properties of the prepared hydrogels were compared against the classic alginate hydrogel used for cell encapsulation. A larger range of material characteristics (from lax to stiff) and better laboratory maneuverability were demonstrated, which permit to design appropriate compositions for particular cell types. All hydrogels undergo fast liquefaction at temperatures between 42 and 50 °C, permitting the cell release after a short innocuous thermal shock.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 169, 1 August 2017, Pages 366-375
نویسندگان
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