کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5157548 1500594 2017 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
PEI-cellulose nanocrystal hybrids as efficient siRNA delivery agents-Synthesis, physicochemical characterization and in vitro evaluation
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
PEI-cellulose nanocrystal hybrids as efficient siRNA delivery agents-Synthesis, physicochemical characterization and in vitro evaluation
چکیده انگلیسی
We describe the synthesis of cellulose nanocrystals (CNCs) complexed to siRNA. PEI (600 Da) was first covalently attached to CNCs by reductive amination reaction, then CNCs-PEI were loaded with siRNA, resulting in the formation of CNCs-PEI-siRNA particles, strongly stabilized by ionic interactions. Efficient cellular uptake of these particles was monitored by ethidium bromide staining. Not only did CNCs-PEI show no cytotoxicity at the studied concentrations, but they also protected siRNA from degradation and favored its delivery into the cells. This siRNA (siRNA killer) is able to silence the expression of cell cycle genes and to induce cell death by apoptosis. Therefore, this study suggests that these CNCs-PEI are promising non-viral nanovehicles for siRNA delivery and for efficient anti-tumor strategy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 164, 15 May 2017, Pages 258-267
نویسندگان
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