High-resolution hyaluronidase inhibition profiling combined with HPLC-HRMS-SPE-NMR for identification of anti-necrosis constituents in Chinese plants used to treat snakebite

Four hyaluronidase inhibitors, i.e., lansiumamide B (6), myricetin 3-O-β-d-glucopyranoside (7), vitexin (8) and 4′,7-dihydroxy-5-methoxyflavone-8-C-β-d-glucopyranoside (9) were identified via high-resolution hyaluronidase inhibition profiling combined with HPLC-HRMS-SPE-NMR.