Purification and characterization of a stable Kunitz trypsin inhibitor from Trigonella foenum-graecum (fenugreek) seeds

TfgKTI is a competitive inhibitor with high binding affinity with (A) trypsin (Ki 3.01 × 10−9 M) and (B) chymotrypsin (Ki 0.52 × 10−9 M).