High level protein-purification allows the unambiguous polypeptide determination of latent isoform PPO4 of mushroom tyrosinase

Mushroom tyrosinase diversity - six precursor isoforms, all capable of being activated and generating protein purification interfering compounds - is taking one step further to reconnaissance. This innovative method for protein purification provides access to latent tyrosinase from natural sources and enabled experiments revealing insights in a hitherto uncharacterized isoform.