Sesquiterpenoids from Chloranthus multistachys

► Nine sesquiterpenoids were isolated and characterized by NMR spectroscopic analysis. ► One is an 8,9-seco-lindenane disesquiterpenoid. ► Absolute configurations of the eight other compounds were characterized further by single-crystal X-ray experiments and CD analysis. ► The cytotoxicity and antifungal of the nine sesquiterpenoids were evaluated, but all were inactive.