Antiviral chlorinated daphnane diterpenoid orthoesters from the bark and wood of Trigonostemon cherrieri

► Ten daphnane diterpenoid orthoesters were isolated from Trigonostemon cherrieri. ► They all possess a mono- or dichlorovinyl group on the daphnane core. ► They were isolated using bioassay-guided and LC/MS-guided fractionations. ► They are a class of non-nucleosidic inhibitor of the DENV NS5 RdRp. ► They showed strong antiviral activity against CHIK, Sindbis and Semliki forest virus.