Anti-hepatitis B virus constituents from the stem bark of Streblus asper

► The constituents of stem bark of Streblus asper were investigated. ► Activity-guided isolation led to discovery of 22 compounds including 7 previously unreported. ► 10 compounds exhibited anti-HBV activities in vitro. ► Magnolol and 9-β-xylopyranosyl-isolariciresinol showed significant anti-HBV activities.