Glucosinolate structures in evolution

► We provide a critical review of reported natural glucosinolate structures. ► A considerable number of suggested structures are insufficiently documented. ► By 2011, around 132 natural glucosinolates were scientifically documented. ► Glucosinolates are specialized metabolites subject to frequent evolution. ► Side chain specific in planta metabolic reactions may influence glucosinolate evolution.