Flavonoid and cardenolide glycosides and a pentacyclic triterpene from the leaves of Nerium oleander and evaluation of cytotoxicity

► Isolation of flavonoids, triterpenes and cardenolides from Nerium oleander leaves. ► Structure elucidation by TOFESIMS and 1D and 2D NMR spectroscopy. ► Cytotoxicity assays of adynerin, oleandrin, odoroside A and B against MCF-7 cells. ► Evaluation of oleandrin, odoroside A and B against 57 cell lines of human cancer. ► First report of cytotoxicity of oleandrin, odoroside A and B against 57 cell lines.