Steroidal glycosides from Ruscus ponticus

► A comparative metabolite profiling of different parts of Ruscus ponticus was obtained. ► HPLC-ESIMSn method proved to be a rapid tool to screen different classes of saponins. ► Eleven saponins, of which two new compounds, were identified in the underground parts. ► Nine new saponins were isolated from R. ponticus leaves. ► Dehydrated furostanol derivatives are reported for the first time in a Ruscus species.