Progesterone 5β-reductase of Erysimumcrepidifolium: cDNA cloning, expression in Escherichia coli, and reduction of enones with the recombinant protein

► The rEcP5βR shares a high degree of sequence and structural identity to Arabidopsis thaliana rAtSt5βR. ► The enone reductase is a highly substrate-promiscuous enzyme. ► Functional enzymes are found in several species. ► Species show phylogenetic and geographic relationships.