Temperature effect on leaf water deuterium enrichment and isotopic fractionation during leaf lipid biosynthesis: Results from controlled growth of C3 and C4 land plants

► Controlled growth experiments were conducted to understand the effect of temperature on leaf water 2H (deuterium) isotope enrichment and hydrogen isotope fractionation during biosynthesis of leaf lipids. ► Change in leaf temperature and/or transpiration may cause change in leaf water flowpath within leaf vasculature, hence affect the laminar 2H isotope enrichment. ► Temperature change from 20 to 30 °C has only negligible effect on hydrogen isotopic fractionation during lipid biosynthesis.