Redirection of tryptophan metabolism in tobacco by ectopic expression of an Arabidopsis indolic glucosinolate biosynthetic gene

► Indole-3-acetaldoxime can be converted to indole-3-acetonitrile in plant species that do not make indole glucosinolates. ► Overexpression of the YUCCA gene does not result in accumulation of the proposed downstream metabolite indole-3-acetaldoxime. ► [2′,2′-2H2]IAN and [13C1]IAN are less reliable internal standards for quantification of endogenous IAN than are [2H5]IAN and [13C6]IAN.