Phosphoproteomics perspective on plant signal transduction and tyrosine phosphorylation

► The plant signal transduction field has evolved rapidly in the last decade. ► Major contributing factors are the advances in (phospho)proteomics methodology. ► Quantitative approaches allow large-scale identification of putative signaling events. ► Tyrosine phosphorylation is shown to be an integral part of plant signal transduction. ► Phosphotyrosine signaling in plants and animals evolved along different lines.