Autophosphorylation profiling of Arabidopsis protein kinases using the cell-free system

► Highly sensitive detection method for autophosphorylation was developed. ► 179 of 759 Arabidopsis protein kinases (PKs) had autophosphorylation activity. ► Four groups (1.6, 1.10, 4.4 and 4.5) were high autophosphorylation PKs. ► High autophosphorylation PKs were involved in diverse biological functions.