A wounding-induced PPO from cowpea (Vigna unguiculata) seedlings

Polyphenol oxidases (Mrs 58, 73 and ≅220 kDa) are induced locally and systemically by wounding in unifoliates of cowpea plants. Purified PPO shows an improved affinity towards small o-diphenols, an optimum pH 6.0 and a high thermal stability. SDS acts as an efficient activator, whereas tropolone serves as an efficient inhibitor for this soluble wounding-induced cowpea PPO.