Pokeweed antiviral protein region Gly209-Lys225 is critical for RNA N-glycosidase activity of the prokaryotic ribosome

We investigated the region of the pokeweed antiviral protein (PAP) that contributes to the RNA N-glycosidase activity towards the prokaryotic ribosome by using a series of chimera proteins between PAP and karasurin. Results indicated that the region of PAP (residues 209-225), which was located in the vicinity of the active site, participates in the substrate specificity.