Flux quantification in central carbon metabolism of Catharanthus roseus hairy roots by 13C labeling and comprehensive bondomer balancing

Metabolic flux quantification is a powerful profiling tool in plant metabolic engineering and systems biology. We introduce the application of “bondomers”, a computationally efficient and intuitively appealing alternative to the commonly used “isotopomers”, toward systemic evaluation of fluxes in central carbon metabolism of Catharanthus roseus hairy roots.