Isolation and characterization of isoinhibitors of the potato protease inhibitor I family from the latex of the rubber trees, Hevea brasiliensis

Centrifuging the latex the rubber tree Hevea brasiliensis separates a rubber fraction (top layer); a clear C-serum fraction (center layer); and a lutoid fraction (bottom layer). Three wound-inducible subtilisin A isoinhibitors of the Potato inhibitor I family were purified from the C-serum and characterized.