Limonoids with Wnt signal inhibitory activity isolated from the fruits of Azadirachta excelsa

Activity-guided isolation of the Azadirachta excelsa MeOH extract using TOP assay led to the isolation of one new (1) and seven known (2-8) limonoids. Compound 4 exhibited TCF/β-catenin transcription inhibitory activity. It showed selective cytotoxicity against HCT116 and AGS which have active Wnt signal.