Isolation and biological activities of lyoniside from rhizomes and stems of Vaccinium myrtillus

► Preparative isolation of lyoniside in only one chromatographic step. ► Occurrence of lyoniside in Vaccinium myrtillus reported for the first time. ► Synergistic action of lyoniside and triterpene acids in allelopathic potential of bilberry. ► Significant antifungal activity of lyoniside against phytopathogens Fusarium oxysporum and Mucor hiemalis.