Investigation of lignan accumulation in developing Linum usitatissimum seeds by immunolocalization and HPLC

► Linum usitatissimum developing seeds were collected and dissected. ► Lignan content increased as a function of the whole seed development stages. ► A stage-depending lignan distribution between seed integuments was quantified by HPLC. ► Lignan distribution was confirmed by immunolocalization.