The preparation and antihyperlipidaemic assay of piperlonguminine in vivo

► Piperlonguminine was synthesised with high yield and purity. ► Synthetic piperlonguminine inhibited body weight gain. ► Synthetic piperlonguminine had significant lipid lowering activities. ► Piperlonguminine is a potential antihyperlipidemic drug candidate. ► Piperlonguminine in Piper longum is too little to be used for pre-clinic and clinic studies.