New lanostane-type triterpenoids, inonotsutriols D, and E, from Inonotus obliquus

► New triterpenoids, inonotsutriols D (1) and E (2) were isolated from I. obliquus. ► The structure of 1 was determined as lanost-8-ene-3β,22R,24R-triol. ► The structure of 2 was determined as lanost-8-ene-3β,22R,24S-triol. ► Cytotoxicities of compounds 1, 3-8 were examined using P388, L1210, HL-60, and KB cell lines. ► Compound 1 exhibited cytotoxic activity against all four cancer cell lines.