کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5180592 | 1502550 | 2015 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: 1H HR-MAS NMR spectroscopy as a simple tool to characterize peptide - Functionalized hydrogels as a function of cross linker density 1H HR-MAS NMR spectroscopy as a simple tool to characterize peptide - Functionalized hydrogels as a function of cross linker density](/preview/png/5180592.png)
- 1H HR-MAS NMR spectroscopy was used to characterize the composition of hydrogels as a function of cross linker densities.
- 1H HR-MAS NMR spectroscopy is a simple tool to quantify bioactive peptide content in cross linked gels.
- NMR results of 1H HR-MAS NMR and 1H NMR spectroscopy are in good agreement for low cross linker densities.
High resolution magic angle spinning (1H HR-MAS) NMR spectroscopy has been used to analyze the composition of a set of bioactive hydrogels. Therefore, a set of six hydrogels with different cross linker densities ranging from 1 to 12Â mol% were prepared by free radical polymerization. The disulfide based cross linkers of the hydrogels were reduced with dithiothreitol (DTT) to generate free sulfhydryl groups giving access to soluble copolymers. Comparison of the 1H NMR results of the soluble copolymers with those obtained from 1H HR-MAS experiments revealed that for lower cross linker densities of 1-2Â mol%, HR-MAS spectroscopy of the hydrogels matches quantitatively the peptide content of the soluble copolymer. With increasing cross linker densities up to 12Â mol% the signal intensity of the aromatic unit is linearly reduced to 62% compared to the results obtained by 1H NMR spectroscopy.
Journal: Polymer - Volume 56, 15 January 2015, Pages 141-146