کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5182588 1380993 2011 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The synthesis and characterization of lanthanide-encoded poly(styrene-co-methacrylic acid) microspheres
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
The synthesis and characterization of lanthanide-encoded poly(styrene-co-methacrylic acid) microspheres
چکیده انگلیسی

Lanthanide-encoded polystyrene microspheres with methacrylic acid (MAA) as a co-monomer and with diameters on the order of 2 μm and a very narrow size distribution were synthesized by two-stage dispersion polymerization (2-DisP). These microspheres were designed as a platform for mass cytometry-based bioassays. Different lanthanides (Ln) were loaded into these microspheres during the synthesis, through the addition of LnCl3 salts and excess MAA to the reaction after about 10% conversion of styrene, i.e., well after the microsphere nucleation stage was complete. Different levels of MAA were employed to investigate the relationship between the number of carboxyl group on the particle surface and the amount of MAA used. The reaction remained well controlled with both 2 and 4 wt % MAA. As monitored by inductively coupled plasma (ICP) mass spectrometry, we found high incorporation efficiency (>95%) of Ln ions into the particles when the total amount of LnCl3 salts in the reaction mixture was sufficiently small. The Ln incorporation efficiency decreased with the increasing amount of LnCl3 salts. Mass cytometry analysis shows that individual microspheres contain ca. 105-108 chelated lanthanide ions, either a single element or a mixture of elements. This method of incorporating lanthanide into P(S-MAA) particles through the second stage of two-stage dispersion polymerization yields microspheres suitable for the highly multiplexed detection of biomolecules.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Polymer - Volume 52, Issue 22, 13 October 2011, Pages 5040-5052
نویسندگان
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