کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5186338 1381102 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Vectorial insertion of bacteriorhodopsin for directed orientation assays in various polymeric biomembranes
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Vectorial insertion of bacteriorhodopsin for directed orientation assays in various polymeric biomembranes
چکیده انگلیسی

Here we demonstrate the rapid reconstitution and preserved functionality of vectorially-inserted bacteriorhodopsin from Halobacterium Halobium in ABA block copolymer thin films. We have utilized a triblock copolymeric membrane that simulates biological systems as a matrix to enable protein refolding to observe the activity of bacteriorhodopsin in purple membrane form as well as in individual molecules. In addition to observing protein-polymer compatibility properties using polymers of varying lengths (4 vs. 8.4 nm in height) and compositions (UV-cross-linkable methacrylate or acrylate ended), we have observed that vectorial insertion of the proteins, and hence, the directionality of proton pumping is dependent upon the pH of the medium to manipulate the asymmetric charge density of the polymer membrane during reconstitution.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Polymer - Volume 47, Issue 9, 19 April 2006, Pages 2935-2941
نویسندگان
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