کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5188527 | 1381158 | 2007 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Availability of biotin incorporated in electrospun PLA fibers for streptavidin binding
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Distribution of biotin at a depth of 3-10Â nm from the surface of electrospun polylactic acid (PLA) fibers has been assessed by X-ray photoelectron spectroscopy (XPS) and compared to the distribution predicted by bulk calculations. Biotin concentration in the outer 3-10Â nm of the fibers is greater than the predicted if biotin was distributed uniformly within the fiber. Availability of biotin for streptavidin binding at the surface of the fibers has been determined via a competitive colorimetric assay. Availability of biotin at the fiber surface was also determined to be greater than predicted by calculations assuming uniform biotin distribution. Additionally, the segregation of biotin to the exterior of the fiber increases disproportionately with increasing overall biotin concentration in the fibers. Confocal microscopy has been used to confirm capture of streptavidin, primary antibodies and fluorescence labeled secondary antibodies on PLA/biotin fibers.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Polymer - Volume 48, Issue 21, 5 October 2007, Pages 6340-6347
Journal: Polymer - Volume 48, Issue 21, 5 October 2007, Pages 6340-6347
نویسندگان
Dapeng Li, Margaret W. Frey, Dionysios Vynias, Antje J. Baeumner,