کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5203390 | 1381929 | 2010 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Current advances in microbial cell factories for lactate-based polyesters driven by lactate-polymerizing enzymes: Towards the further creation of new LA-based polyesters
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
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![عکس صفحه اول مقاله: Current advances in microbial cell factories for lactate-based polyesters driven by lactate-polymerizing enzymes: Towards the further creation of new LA-based polyesters Current advances in microbial cell factories for lactate-based polyesters driven by lactate-polymerizing enzymes: Towards the further creation of new LA-based polyesters](/preview/png/5203390.png)
چکیده انگلیسی
Polylactic acid (PLA), a representative bio-based polyester, has been commonly synthesized via a multi-step by chemical process. The current modes of generating PLA involve microbial fermentation of starting material, lactic acid (LA), followed by chemical ring-opening polymerization. Recently, one-pot complete bioprocess for LA-based polyesters has been established as a microbial cell factory (MCF). The concept is a process conversion from the usual chemical factory to the MCF. This new challenge was triggered by discovery of an engineered LA-polymerizing enzyme (LPE). The LPE was found as one of the members of an extensive mutant library that has been created through the long-term evolutionary engineering study of natural biopolyester-synthesizing enzymes. Needless to say, a strategic method of getting the beneficial mutation in the enzyme is of the utmost importance and an essential step towards accomplishing the desired purpose, the acquisition of the LA-polymerizing activity in this case. In this review, the structures and properties of LPE-catalyzed polymerization products will be discussed as well as backgrounds on establishment of the MCFs for synthesis of LA-based polyesters. Also, experimental strategies for enrichment of the LA fraction will be proposed to further advance the prototype of MCF based on the related metabolic pathways.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Polymer Degradation and Stability - Volume 95, Issue 8, August 2010, Pages 1421-1428
Journal: Polymer Degradation and Stability - Volume 95, Issue 8, August 2010, Pages 1421-1428
نویسندگان
Seiichi Taguchi,