کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5203914 1381944 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Self-assemblies of enzymatically degradable amphiphilic oligopeptides as nonviral gene carrier
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Self-assemblies of enzymatically degradable amphiphilic oligopeptides as nonviral gene carrier
چکیده انگلیسی

Novel biodegradable oligopeptide-type gene carriers composed of cationic residues (KRRRKRKRRRKRKRRC) and oligo leucine segments were developed. The amphiphilic carrier was found to form micelle-like assemblies in aqueous solutions, when the oligo leucine is 12 amino acids length (Pep-L12). NMR, CMC, and GPC analysis revealed their hydrophobic/cationic core/shell morphology. Hydrophobic interaction between leucines is thought to be the major driving force behind formations of assemblies. The transient expression of luciferase introduced to COS-1 cells using Pep-L12 below the CMC is as low as that by the control cationic peptides without leucine residue (Pep-L0), while improved transgene expression was observed in the case of Pep-L12 above CMC. The self-assembly raised the apparent molecular weight and gene transfection ability without loosening their low cytotoxicity. These results indicate that the amphiphilic oligopeptides are very promising materials as highly efficient and less toxic gene carriers.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Polymer Degradation and Stability - Volume 94, Issue 9, September 2009, Pages 1349-1353
نویسندگان
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