کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5209823 | 1503073 | 2015 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Adsorption and recognition of protein molecular imprinted calcium alginate/polyacrylamide hydrogel film with good regeneration performance and high toughness
ترجمه فارسی عنوان
جذب و شناسایی پروتئین هیدروژل آلژینات کلسیم / پلی آکریل آمید با پروتئین مولکولی با عملکرد خوب بازسازی و چقرمگی بالا
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کلمات کلیدی
واردات مولکولی پروتئین، آلژینات کلسیم / پلی آکریل آمید، فیلم هیدروژل جذب، به رسمیت شناختن، فرهنگ سلولی،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
چکیده انگلیسی
Protein imprinted calcium alginate/polyacrylamide hydrogel film (CA/PAM MIP) with high toughness was prepared using bovine serum albumin (BSA) as template molecule, sodium alginate and acrylamide as functional monomers, N,Nâ²-methylenebisacrylamide (MBAA) as the covalent cross-linker and CaCl2 as the ionic cross-linker via UV radiation-reduced polymerization. Factors affecting the adsorption capacity and imprinting efficiency of the BSA-imprinted CA/PAM hydrogel films were investigated, such as ratio of polyacrylamide/sodium alginate, film thickness, MBAA concentration and CaCl2 concentration. Results showed that the CA/PAM MIP exhibited an obvious improvement in terms of adsorption capacity for BSA compared with non-imprinted polymer (NIP). The adsorption capacity of MIP for BSA reached 22.49Â mg/g, which was 2.7Â times higher than NIP. The regeneration property of the BSA-imprinted CA/PAM hydrogel was distinctly improved and the imprinting efficiency of CA/PAM MIP maintained 77.95% of the initial value after five repetitions. Single and binary proteins rebinding indicated that the CA/PAM MIP exhibited good recognition performance. Cell culture experiments showed CA/PAM MIP was more suitable for cell culture than CA/PAM NIP. The residual sodium dodecyl sulfate (SDS) in the elution process leaded to the death of mouse fibroblast cells (L929) after 3Â days. A moderate elution solution without residue eluent should be used to prepare MIP for cell culture.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Reactive and Functional Polymers - Volume 87, February 2015, Pages 7-14
Journal: Reactive and Functional Polymers - Volume 87, February 2015, Pages 7-14
نویسندگان
Kongyin Zhao, Tian Chen, Beibei Lin, Wenkui Cui, Bohong Kan, Ning Yang, Xiangyu Zhou, Xinxin Zhang, Junfu Wei,