کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5210237 | 1382882 | 2013 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Promoted cell proliferation and mechanical relaxation of nanocomposite hydrogels prepared in cell culture medium
ترجمه فارسی عنوان
ترویج تکثیر سلولی و آرام سازی مکانیکی هیدروژل های نانو کامپوزیت تهیه شده در محیط کشت سلولی
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
چکیده انگلیسی
Nanocomposite hydrogels (NC gels) were synthesized through in situ polymerization of N-isopropylacrylamide (NIPAm) in the hectorite clay suspension made from cell culture medium Dulbecco's modified Eagle medium (DMEM). Cell cultured on these NC gels (D-NC gels) surface proliferated faster compared with that on the NC gels synthesized in water due to the nutrients in the D-NC gels. Cells attached and proliferated faster on the D-NC gels with higher modulus. In addition, cell sheets with good viability spontaneously detached from the gel surface by lowering temperature. Their tensile elongation at break was higher than 7 and the true strength Ïtrue was up to 800 kPa. The stress-strain curves of the D-NC gels were described quantitatively with the Mooney-Rivlin equation. A stretched exponential stress equation was adopted to express the stress relaxation of the D-NC gels with an average relaxation time Ï (â¼102 s) estimated from data fitting, showing a broad distribution (polydispersity k â¼Â 0.4). The Ï value, which was used in simulation for the tensile creep compliance of the D-NC gels, became shorter with increasing clay concentration due to the decrease in the network chain length. The relaxation process was similar to the natural articular cartilage. This work provides a facile way to produce hydrogels with improved cell compatibility and satisfactory mechanical properties for biomedical applications.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Reactive and Functional Polymers - Volume 73, Issue 5, May 2013, Pages 683-689
Journal: Reactive and Functional Polymers - Volume 73, Issue 5, May 2013, Pages 683-689
نویسندگان
Tao Wang, Weixiang Sun, Xinxing Liu, Chaoyang Wang, Shiyu Fu, Zhen Tong,