کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5210240 1382882 2013 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Synthesis, characterization, and properties of novel hydrophobically associating fluorinated copolymers for DNA delivery
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Synthesis, characterization, and properties of novel hydrophobically associating fluorinated copolymers for DNA delivery
چکیده انگلیسی
Novel fluorinated associative copolymers poly(ethylene glycol)-b-poly(2-(perfluorohexyl)ethyl methacrylate (PFHEMA)-co-3-[N-(2-methacroyloylethyl)-N,N-dimethylammonio]-propane sulfonate (DMAPS)) (PEG-b-PPFHEMA/PDMAPS) were prepared as a type of non-viral gene vector. The series of PEG-b-PFHEMA/DMAPS with different molecular weights and compositions were characterized by gel permeation chromatography-multi-angle light scattering, 1H NMR, 19F NMR, and elemental analysis. The interactions of the copolymers with calf thymus DNA and the morphologies of the resulting complexes were studied by fluorescence spectroscopy, dynamic light scattering, and transmission electron microscopy. In addition, the DNA-polymer interaction, as determined by the gel retardation assay, and the low cytotoxicity of PEG-b-PPFHEMA/PDMAPS demonstrated that these copolymers have good DNA binding capacity and potential for high performance in the practical application of DNA delivery.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Reactive and Functional Polymers - Volume 73, Issue 5, May 2013, Pages 703-709
نویسندگان
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