کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5210496 | 1382896 | 2012 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
New method for the synthesis and purification of branched mPEG2lys
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: New method for the synthesis and purification of branched mPEG2lys New method for the synthesis and purification of branched mPEG2lys](/preview/png/5210496.png)
چکیده انگلیسی
Branched PEG structures are particularly useful for the pegylation of different biomaterials. In this regard, the branched structure bearing a lysine core and two mPEG chains linked by urethane bonds (mPEG2lys) has been used for the pegylation of several important biomolecules. In this work we describe the preparation of a new functionalized mPEG featuring an alkoxy-carbonylimidazolium iodide reactive group that can be used for the urethane bonds formation required in mPEG2lys (20 kDa). The procedure for the preparation of the mPEG-alkoxy-carbonylimidazolium iodide involves the initial reaction of mPEG with N,Nâ²-carbonylbismidazole (CDI) as phosgene equivalent, followed by alkylation with methyl iodide. The functionalized mPEG was reacted with a silylated lysine derivative affording mPEG2lys in a single step. Therefore, harmful reagents (like phosgene derivatives) are not employed, making the process safe and straightforward. A 2-step purification procedure of mPEG2lys is also presented. It is noteworthy that by following this method pure mPEG2lys was obtained with 41% isolated yield. The mPEG2lys was further converted to the N-hydroxysuccinimidyl ester by diimide activation and successfully used in the pegylation of interferon α-2a.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Reactive and Functional Polymers - Volume 72, Issue 1, January 2012, Pages 107-113
Journal: Reactive and Functional Polymers - Volume 72, Issue 1, January 2012, Pages 107-113
نویسندگان
Marianela González, Ricardo J.A. Grau, Santiago E. Vaillard,