کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5210739 1382913 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
m-Aminophenylboronic acid as a functional monomer for fabricating molecularly imprinted polymer for the recognition of bovine serum albumin
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
m-Aminophenylboronic acid as a functional monomer for fabricating molecularly imprinted polymer for the recognition of bovine serum albumin
چکیده انگلیسی
Using m-aminophenylboronic acid (APBA) as a functional monomer, molecularly imprinted polymer (MIP) imprinted with bovine serum albumin (BSA) was fabricated on activated glass spheres under optimized conditions. Key factors in the prepolymerization reaction (between APBA and BSA), such as buffer pH, ionic strength and reaction time, were carefully optimized as previously reported [H.F. Wang, W.Y. Li, X.W. He, et al. Acta Chim. Sinica 65 (2007) 43-48]. The interaction between APBA and BSA during the prepolymerization stage was investigated and optimized, and ideal conditions for protein rebinding experiments were determined as well. Protein rebinding and enriching properties of polymers were studied in single and competitive binding protocols, respectively. The key point of the present paper is that the binding selectivity of polymers may be estimated by the amount of bound-protein recovered from a protein-saturated polymer. Results demonstrated that the selectivity of MIP towards its template protein is superior to that of non-imprinted polymer (NIP).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Reactive and Functional Polymers - Volume 68, Issue 9, September 2008, Pages 1291-1296
نویسندگان
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