کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5210768 1382914 2009 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
New platforms for 3-D microarrays: Synthesis of hydrophilic polymethacrylate monoliths using macromolecular porogens
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
New platforms for 3-D microarrays: Synthesis of hydrophilic polymethacrylate monoliths using macromolecular porogens
چکیده انگلیسی

Hydrophilic macroporous monolithic material based on poly(glycidyl methacrylate-co-glycerol dimethacrylate) was synthesized by photo-initiated free-radical polymerization. Different pore-forming agents, including low molecular mass cyclohexanol and dodecanol, as well as the solutions of hydrophobic polymers, namely, polystyrene in toluene and poly(dimethyl siloxane) in hexane, were used to obtain the macroporous polymer platforms intended for new type of 3-D microarrays (biochips). The porous characteristics of functional copolymers obtained were investigated by mercury intrusion porosimetry and scanning electron microscopy. Some of developed materials were tested for protein microarray construction. Demonstration of potential of suggested materials, as well as optimization of protein covalent immobilization conditions, were realized using model mouse IgG - goat anti-mouse IgG affinity pair.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Reactive and Functional Polymers - Volume 69, Issue 6, June 2009, Pages 385-392
نویسندگان
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