Furospirostanol and spirostanol saponins from the rhizome of Tupistra chinensis and their cytotoxic and anti-inflammatory activities

Five new furospirostanol saponins (1–5), four new spirostanol saponins (6–9), along with one known spirostanol saponin were isolated from the rhizome of Tupistra chinensis. The chemical structures were determined by spectroscopic and chemical methods, including IR, NMR, MS, and GC analyses. The antiproliferative effects against five human cancer cell lines were assayed for all the isolated compounds. Compounds 6–10 showed potent cytotoxic activities against the five cancer cell lines. The isolated compounds were evaluated the inhibitory activities on nitric oxide (NO) production induced by lipopolysaccharide in a macrophage cell line RAW 264.7. Compounds 6–10 showed significant inhibition on NO production with IC50 values between 3.1 and 4.4 μM.

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