کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5216812 1383277 2014 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
β-Biguanidinium-cyclodextrin: a supramolecular mimic of mitochondrial ADP/ATP carrier protein
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
β-Biguanidinium-cyclodextrin: a supramolecular mimic of mitochondrial ADP/ATP carrier protein
چکیده انگلیسی

We reported a novel mono-β-cyclodextrin derivative, mono-6-deoxy-6-biguanidino-β-cyclodextrin (β-biGCD), which was investigated as a mimic of ADP/ATP carrier (AAC). Its affinity toward AMP, ADP, and ATP was evaluated by means of isothermal titration calorimetry (ITC). The association constants (Ka) of β-biGCD binding to AMP, ADP, and ATP were determined to be (1.07±0.04)×106, (5.86±0.02)×106, and (4.33±0.06)×106 L mol−1, respectively, which were 100-fold higher than mono-guanidino-β-cyclodextrin (ca. 104 L mol−1). UV spectroscopic titrations further confirmed the above results. The interaction between β-biGCD and nucleotides was probed by docking simulation. These results reveal that the biguanidinium moiety mimics the arginine residues of mitochondrial AAC protein.

265Modified β-cyclodextrin with a cationic biguanidyl group exhibits high binding affinity toward AMP, ADP, and ATP in water, which mimic the function of mitochondria ADP/ATP carrier protein (AAC). The cationic biguanidyl group plays a role analogue to the Arg residues of mitochondrial AAC.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Tetrahedron - Volume 70, Issue 14, 8 April 2014, Pages 2378-2382
نویسندگان
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