کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5218514 | 1383330 | 2012 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development of a high-throughput screening method for racemase activity and its application to the identification of alanine racemase variants with activity towards l-arginine
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Development of a high-throughput screening method for racemase activity and its application to the identification of alanine racemase variants with activity towards l-arginine Development of a high-throughput screening method for racemase activity and its application to the identification of alanine racemase variants with activity towards l-arginine](/preview/png/5218514.png)
چکیده انگلیسی
A high-throughput solid phase screening method has been developed for alanine racemase. Conversion of an l-amino acid to the d-amino acid is detected by a d-amino acid oxidase, which is utilised as a reporter enzyme along with horseradish peroxidase and a colourimetric dye. This system has successfully been used to identify a mutant with increased activity towards arginine. Kinetic analysis and modelling studies have been performed to understand the effect of the mutations found.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Tetrahedron - Volume 68, Issue 37, 16 September 2012, Pages 7564-7567
Journal: Tetrahedron - Volume 68, Issue 37, 16 September 2012, Pages 7564-7567
نویسندگان
Simon C. Willies, Jemma L. White, Nicholas J. Turner,