Highly specific-glucose fluorescence sensing based on boronic anthraquinone derivatives via the GOx enzymatic reaction

The boronic acid anthraquinones, oHAQB and pHAQB, have been designed, and demonstrated to serve as fluorogenic biosensors for glucose. The sensory molecule, oHAQB, has exhibited the specific-glucose sensing via the GOx enzymatic reaction. In this contribution, the fluorescence changes of oHAQB reasonably correspond to the concentration of glucose upon the conversion of boronic acid to hydroxy based sensor by H2O2 generated from glucose along with GOx enzymatic reaction. Our sensing ensemble was then successfully applied to determine the glucose concentration in the range of 0.08-0.42 mM. The limit of detection (LOD) of oHAQB for glucose detection using the GOx enzymatic probe is approximately 0.011 mM.