Synthesis of dendritic oligodeoxyribonucleotide analogs with nonionic diisopropylsilyl linkage

Series of new dendritic oligodeoxyribonucleotide analogs with diisopropylsilyl linkage were prepared via liquid-phase synthesis. Dendron was employed as soluble support, which facilitated purification of the products by simple solvent precipitation. Sequences leading to the dendritic octamer 5′-TSiTSiTSiTSiTSiTSiTSiTSiD2-3′ (T8D2) were prepared and each isolated intermediate was characterized by NMR and mass spectrometry. The recognition properties of two complementary dendritic dinucleotide analogs of TSiTSiD2 (T2D2) and ASiASiD2 (A2D2) were also studied by NMR and FT-MS spectra in a nonaqueous aprotic environment.

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