کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5220649 | 1383395 | 2012 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: DNA-directed formation of peptide bond: a model study toward DNA-programmed peptide ligation DNA-directed formation of peptide bond: a model study toward DNA-programmed peptide ligation](/preview/png/5220649.png)
A model study of DNA-directed peptide ligation has been developed by transferring fluorescent reporting group from small molecule thioester to a DNA strand (template DNA) in the presence of a thiol-functionalized DNA strand (auxiliary DNA), mimicking the Native Chemical Ligation (NCL) reaction. This DNA-directed transfer shows dependence on the sequence complementarity of the two DNA strands, with in situ generated 4-thiolphenylmethyl functionalized oligonucleotide as the auxiliary DNA strand, under mild basic condition (pH=8.5), and with tris(2-carboxyethyl) phosphine hydrochloride (TCEP) as a reducing agent. Reactions with different amino acid α-thioesters resulted in varied transfer efficiencies from glycine to α-substituted amino acids. This study has provided the basic foundation to use DNA-programmed chemistry toward the chemical synthesis or unnatural modification of protein molecules.
Journal: Tetrahedron - Volume 68, Issue 26, 1 July 2012, Pages 5152-5156