Synthetic receptor and its association with CTD peptides

In this study, a receptor for phosphorylated peptide was developed. The receptor contains Zn2+-dipicolylamine (Zn-Dpa) moiety at one end and guanidiniocarbonyl pyrrole at the other. The association between the receptor and CTD (carboxy-terminal domain of RNA polymerase II) peptides with different phosphorylated patterns was investigated by isothermal titration calorimetry and molecular docking, and the receptor showed much higher affinity towards bis-phosphorylated CTD peptide with affinity constant K=1.09×105.