کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5273129 1385482 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Two-step enzymatic modification of solid-supported bergenin in aqueous and organic media
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Two-step enzymatic modification of solid-supported bergenin in aqueous and organic media
چکیده انگلیسی

The natural flavonoid bergenin was directly immobilized onto carboxylic acid-functionalized controlled pore glass (carboxy-CPG) at 95% yield. Immobilized bergenin was brominated via chloroperoxidase in aqueous solution and then transesterified with vinyl butyrate in diisopropyl ether by subtilisin carslberg (SC) extracted into the organic solvent via ion-pairing. Enzymatic cleavage of 7-bromo-4-butyrylbergenin from carboxy-CPG (9.6% final yield) was accomplished using lipase B (LipB) in an aqueous/organic mixture (90/10 v/v of water/acetonitrile), demonstrating the feasibility of solid-phase biocatalysis of a natural product in aqueous and non-aqueous media.

A natural flavonoid molecule, bergenin is attached to a solid support without a linker and is modified and cleaved enzymatically in both aqueous and organic media as a demonstration of solid-phase biocatalysis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Tetrahedron Letters - Volume 51, Issue 8, 24 February 2010, Pages 1220-1225
نویسندگان
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