کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5273129 | 1385482 | 2010 | 6 صفحه PDF | دانلود رایگان |
The natural flavonoid bergenin was directly immobilized onto carboxylic acid-functionalized controlled pore glass (carboxy-CPG) at 95% yield. Immobilized bergenin was brominated via chloroperoxidase in aqueous solution and then transesterified with vinyl butyrate in diisopropyl ether by subtilisin carslberg (SC) extracted into the organic solvent via ion-pairing. Enzymatic cleavage of 7-bromo-4-butyrylbergenin from carboxy-CPG (9.6% final yield) was accomplished using lipase B (LipB) in an aqueous/organic mixture (90/10 v/v of water/acetonitrile), demonstrating the feasibility of solid-phase biocatalysis of a natural product in aqueous and non-aqueous media.
A natural flavonoid molecule, bergenin is attached to a solid support without a linker and is modified and cleaved enzymatically in both aqueous and organic media as a demonstration of solid-phase biocatalysis.
Journal: Tetrahedron Letters - Volume 51, Issue 8, 24 February 2010, Pages 1220-1225