کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5273300 1385487 2013 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A substrate for the detection of broad specificity α-l-arabinofuranosidases with indirect release of a chromogenic group
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
A substrate for the detection of broad specificity α-l-arabinofuranosidases with indirect release of a chromogenic group
چکیده انگلیسی

The synthesis of a compound containing a 4-nitrocatechol bound to two vicinal α-l-arabinofuranosyl moieties through a linker arm was achieved using a sulfate protecting group to facilitate selective alkylation of one aromatic hydroxyl. Several α-l-arabinofuranosidases displaying different selectivities were tested and a simple microtiter plate-based assay was developed. The observed resistance of the compound to α-l-arabinofuranosidase-mediated hydrolysis makes it suitable for the identification of enzymes that are able to accommodate bis-arabinofuranosylated moieties.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Tetrahedron Letters - Volume 54, Issue 24, 12 June 2013, Pages 3063-3066
نویسندگان
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