Affinity purification of the key enzyme of nyctinasty controlling the rhythm of leaf movement using gluconamidine ligand

Synthesis of affinity gel aimed for leaf-opening factor β-glucosidase (LOFG) and affinity purification of LOFG are presented. Gluconamidine-based β-glucosidase inhibitor was utilized for the ligand of the affinity gel. β-Glucosidase exhibiting activity shift throughout the day was selectively purified from Lespedeza cuneata Don by the affinity gel. The resulting LOFG exhibited a high substrate specificity toward the leaf-opening factor.