Synthesis and biochemical properties of oligodeoxynucleotides acylated by the chemically stable 2-(trimethylsilyl)benzoyl (TMSBz) group at the 5′ or 3′ terminus

Oligodeoxynucleotides acylated with a 2-(trimethylsilyl)benzoyl (TMSBz) group at the 5′ or 3′ terminus were synthesized according to the general method used for DNA synthesis. The acylated DNA oligomers could be easily purified due to the high lipophilicity of the TMSBz group and showed enhanced hybridization ability and resistance to exonucleases.

Oligonucleotides acylated by the chemically stable TMSBz group at their 5′ or 3′ terminus were synthesized. This modification enhanced not only their resistance to exonucleases but also hybridization affinity for the complementary DNA oligomers.