Detection of genetic polymorphisms with high sensitivity by DNA-perylene conjugate

Modified oligodeoxyribonucleotides (ODNs) involving two perylene moieties are synthesized. By using this ODN, one-base deletion can easily be distinguished with high sensitivity. In addition, emission color of the solution greatly changed so that the detection was possible even by naked eyes.

Perylene-modified oligodeoxyribonucleotides are synthesized for the detection of deletion polymorphisms with high sensitivity on the basis of the change of monomer → excimer emission.