Promiscuous acylase-catalyzed aza-Michael additions of aromatic N-heterocycles in organic solvent

An efficient protocol for aza-Michael addition of aromatic N-heterocycles to α,β-unsaturated compounds has been described. The reaction was catalyzed by promiscuous zinc-active-site acylase in organic solvent at 50 °C and most of the procedures could provide products in good yields in several hours (0.5-6 h).